Exploring temperature-dependent transcriptomic adaptations in Yersinia pestis using direct cDNA sequencing by Oxford Nanopore Technologies

Link

Publication webpage.

Significance

This study introduces a PCR-free, long-read RNA-Seq workflow for Yersinia pestis, uncovering temperature-driven transcriptional adaptations and novel operons (including within the pathogenicity island) through an approach fully suited to high-risk pathogens.